Unexpected problems often occur in HE staining. One of the most common problems is that tissue will fall off the slide. This not only causes the waste of materials, but also causes the waste of a large number of hours in the early stage of sampling, fixing, slicing, etc. To this end, Beant combined with the practical experience of a number of experts, will fall off the reasons sorted out as follows for your reference.

1. Slice too thick

Suggestion: We can make the slices evenly thick, and the thickness is between 3-5um.

2. Low baking temperature and short baking time

Suggestion: We can adjust the baking temperature and time, 20-30min.

3. The baking temperature is too high and the time is too long

As a result, the tissue section is scorched, contracted and deformed, wrinkled and easy to fall off.

Suggestion: We can adjust the temperature and time of the baking sheet.

4. Organization itself

Such as clots, thrombus, dry or hard tissue and broken tissue, easy to fall off when the section is dyed.

Suggestion: We can use anti-slip slide to solve the problem.

5. Tissue dehydration and lack of transparency

The paraffin wax can not be immersed, the tissue is soft, the section shrinks when dewaxing, and the water expands and falls off.

Suggestion: We can adjust the time of tissue fixation, dehydration and transparent wax dipping.

6. Tissue dehydration and excessive transparency

Wax immersion time is too long or the temperature is too high, resulting in sharp contraction of the tissue, hard, too brittle resulting in incomplete sections and easy to fall off.

Suggestion: We can use ethanol and glycerin half soak the section before slicing.

7. Display water temperature is too low

The section has wrinkles and does not flatten or the tissue surface has bubbles when the slice is pulled out

Suggestion: We can adjust the water temperature and empty the bubbles when fishing.

8. The slide is not clean

There is oil or dust on the surface of the slice.

Suggestion: We can replace the clean slide.

9. Take off the tablet

After dewaxing without high to low concentration of ethanol into the water, flushing too fast or shaking too much, it is easy to make the section fall off.

Suggestion: We need to install dye solution technology operating procedures required operation.

10. The pH of dyeing solution or reagent is not suitable

Differentiation fluid or anti-blue fluid is too strong, can also lead to slicing off.

Suggestion: We can replace the standardized, commercial dyeing solution and reagent.

11. Improper dyeing or poor dyeing effect

Returning to re-staining may cause the section to fall off.

Suggestion: When dyeing initially, we can operate according to the requirements of the dyeing technical operating procedures.

12. Dyeing solution or dyeing vessel is unclean

Suggestion: Before operation, we can filter the dye and clean the ware.

        In summary, there are many reasons for the detachment of sections of HE stained tissue, including non-staining technique problems, some of which are caused by improper treatment of sampling, fixation, dehydration, transparency and section before staining. So how can we have a greater chance of making a successful HE stain slice? Please try Beyant HE HD constant dye solution.

        HE HD Constant dyeing solution is a product that can help you achieve the standardization of HE dyeing. It is composed of stabilization solution, hematoxylin dyeing solution, eosin dyeing solution, anti-blue solution and differentiation solution, and does not contain toxic and harmful reagents such as mercury chloride and formic acid. The dyeing effect is clear and bright, and the core and substance are distinct, and the dyeing color is stable and durable, which can ensure the consistent dyeing effect of the first piece to 5000 pieces.

Dyeing effect display