HE sections will fade after 1-2 years of storage, which will directly affect the doctor's observation results. The reasons for the long-term preservation and fading of HE sections are related to the properties of dye solution, preparation process, sealing agent, humidity and sunlight. Although one or two patches can be solved, large batches of patches are not practical. Common causes of fading can be summarized as follows:
(1) The pH of the fixed solution is not suitable, and the tissues fixed for a long time, especially the tissues fixed with acidic fixing solution, are not washed or washed adequately before dehydration, and are easy to fade after dyeing.

(2) The pH of the dyeing solution and the reagent used is not suitable for a very common and important reason. In HE staining, the use of excessively alkaline solution to blue, easy to cause the fading of cytoplasm, if the use of pH weakly alkaline solution to blue and rinse with tap water, it is not easy to cause the section fading.

(3) If the dye promoting agent glacial acetic acid is too much when preparing eosin dye solution, it will produce a large number of bubbles floating on the liquid surface, which is not easy to disappear for a long time, the dye solution is easy to turbidity, and the supernatant is a light powder color, indicating that the dye solution failure is easy to fade even if it is stained with color.

Reference improvement scheme

• The effect of water back blue is better than that of using alkaline liquid back blue liquid, the core/pulp contrast is high, and the preservation of a long time has less fading or even no fading. Or use commercial professional dyeing solution, such as Beant HE HD constant dyeing solution, the blue return effect is good, the dyeing effect is constant and stable.
  
  The time and concentration of differentiation should be controlled, and the differentiation solution will decolorize the overstained nuclei and the cytoplasmic components that should not be stained.
  
  After differentiation, it should be fully washed with running water to avoid the residual acidic substances in the slices, resulting in a long time in the acidic weak differentiation environment.
  
  Eosin is an acidic mixed solution, and the acidic environment should be removed as far as possible after eosin staining

• Sealing plate

• • The seal is not strict, and there is a lot of water or small bubbles mixed in the tissue section
    
    The sealing glue is too thin, and it is easy to cause tissue exposure after drying
    
    The PH value of the sealing glue is not suitable, and neutral gum should be used
    
    The dehydration before sealing is not clean, and the alcohol is exposed to the air for a long time to absorb water
    
    HE film was exposed to light for a long time

  • • save

    • Avoid high temperature and high humidity environment as much as possible
      
      Use a professional pathology section storage cabinet
      
      It is possible to place the archive room independently

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      HE section fading will cause a lot of inconvenience, but if you use BeANT HE HD constant dyeing solution, and through the dyeing scheme provided by professional reagent engineers, avoid the above practices, you can achieve stable and constant dyeing effect, and use neutral gum sealing sheet for a long time. A litre HE set can dye 1-5000 pieces consistently

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